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Assurance GDS® for Top STEC (eae)

Speed and Accuracy
Rapid accurate detection method features the latest innovations in genetic detection technology and food microbiology for the detection of Top STEC, including E. coli (O157:H7, O26, O45, O103, O111, O121, and O145.)

Three Levels of Specificity
The versatile collection of Assurance GDS STEC assays incorporate three levels of specificity, including proprietary PickPen® Immunomagnetic Separation (IMS) to ensure fast and accurate time to results.

With the PickPen IMS, magnetic particles coated with antibodies specific to the Top STEC O-groups are combined with the enriched sample. The PickPen device collects and removes the particles with the bound E. coli separating them from the non-target O groups and helping to ensure detection of the gene targets is limited to the desired O-groups of E. coli.

Conducting IMS prior to analysis by PCR minimizes false positives caused by non-target O-groups of E. coli.

Assurance GDS for Top 6 and 7 STEC Features

  • Sequential approach to detecting Top STEC.  Following sample preparation with IMS against the Top 6 or Top 7 O groups, samples are analyzed for the presence of (eae) with the GDS Top 6 or 7 STEC (eae) assay.  Positive samples are subsequently analyzed for Shiga Toxin genes with the Assurance GDS Shiga Toxin Genes (Top 6 or Top 7) assay.
  • Available for Top 6 STEC and Top 7 STEC (includes E. coli O157:H7)
  • Single-step 10-18 h enrichment in mEHEC®, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

Assurance GDS® for Top STEC (eae) Details:


(eae) gene in Top 6/7 STEC Serogroups
Top 6/7: USDA FSIS Letter of No Objection                                                                          Top 7: AOAC Performance Tested Method 071303
Companion Products
Assurance GDS MPX Top 7 STEC
Assurance GDS MPX Top 6 STEC
Assurance GDS E. coli O157:H7 Tq
Assurance GDS Shiga Toxin Genes (O157) Tq
Assurance GDS Shiga Toxin Genes (Top 6) Tq
Assurance GDS Shiga Toxin Genes (Top 7) Tq
Polymerase Chain Reaction
2 - 8 °C
Assurance GDS Top 6 STEC (eae) Tq  71013-100 100 tests
Assurance GDS Top 7 STEC (eae) Tq  71017-100  100 tests
Additional Information
About the Target